The protein level of re-expressed HER3 kept increasing in the 6 h under treatment of wortmannin (10 μM) in NOTCH1-knockdown SCCHN cells, in contrast, the protein of HER3 dwindled to less than 50% of the original level in the DMSO-treated control group (Fig. 3A). This evidence concerns the gene NOTCH1 and head and neck squamous cell carcinoma.