The lysosome-related pathologies, together with neuron loss and Aβ plaque deposition, have been shown to be accentuated in EOFAD due to mutations in PSEN1.46 We tested the protein levels of lysosomal markers LAMP1 and LAMP2A (lysosomal-associated membrane protein 2), autophagy markers MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3) and SQSTM1 (sequestosome 1), and synaptic markers DLG4/PSD95 (Discs large MAGUK scaffold protein 4) and SYP (synaptophysin) in cells overexpressing ACAA1 p.N299S compared to those of cells overexpressing ACAA1 WT. The gene discussed is ACAA1; the disease is early-onset autosomal dominant Alzheimer disease.