We investigated whether ACAA1 p.N299S would play a vital role in AD pathogenesis by using the adeno-associated virus (AAV php.eb) vector-mediated overexpression of ACAA1 WT and p.N299S in APPswe/PSEN1dE9 (APP/PS1ΔE9) mice. The gene discussed is APP; the disease is Alzheimer disease.