The three non-AD family members had the APOE ε4 allele, but the AD proband was APOE ε4 negative and had nine potentially damaging variants (ACAA1 p.N299S, TET2 p.E1151*, TBC1D3D p.K25*, PSG4 p.Y351*, OR4X2 p.Y27*, SLC6A18 p.Y319*, GEMIN8 p.E195V, DMD p.K1510R, and GPR112 p.P368H); each variant had a genotype different from that of other non-AD members (Supplementaty Table S2). This evidence concerns the gene APOE and Alzheimer disease.