We observed that inhibition of HIF-1α significantly suppressed BNIP3 expression and the administration of YC-1 and 3-MA alone or in combination significantly downregulated the expression of autophagy-related genes LC3II, Beclin1, and HMGB1, and increased the expression level of P62 when exposed to mild hyperthermia, hypoxia, and nutrition deficiency microenvironment. Here, HMGB1 is linked to nutritional deficiency disease.