To further investigate the correlation between hyperthermia-induced the HIF-1α/BNIP3/Beclin1 autophagy signaling pathway in hypoxia and starvation tumor microenvironment, YC-1 was used to suppress the HIF-1α-mediated autophagy pathway, and 3-MA was used for direct inhibition of autophagy. This evidence concerns the gene BECN1 and neoplasm.