We observed that inhibition of HIF-1α significantly suppressed BNIP3 expression and the administration of YC-1 and 3-MA alone or in combination significantly downregulated the expression of autophagy-related genes LC3II, Beclin1, and HMGB1, and increased the expression level of P62 when exposed to mild hyperthermia, hypoxia, and nutrition deficiency microenvironment. The gene discussed is SQSTM1; the disease is nutritional deficiency disease.