This study analysed the levels of amylase, lipase, triglyceride, free fatty acids (FFAs), cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and γ-glutamyl transferase (γ-GT) in the gallbladder bile of gallstone patients, with the aim of providing new evidence for the correlation between PBR and gallstone formation. This evidence concerns the gene GPT and gallstones.