Figure 5C showed that the protein levels of TIMP-1 and TIMP-2 increased to 1.60-fold and 1.85-fold over control in the MCT-treated rats (P < 0.05), respectively; whereas treatment of MCT-induced PAH rats with MS-275 decreased TIMP-1/2 protein levels to 0.92-/0.90-fold over control (P < 0.05 versus MCT-treated group), and administration of miR-34a agomiR reduced TIMP-1/2 protein levels in MCT-treated rats to 0.85-/0.92-fold over control (P < 0.05 versus MCT group). This evidence concerns the gene TIMP1 and pulmonary arterial hypertension.