Interestingly, we observed an enhanced and prolonged suppression of food intake and body weight in DIO PrlhNTS-OX mice (Fig. 4E, F), and found that the overexpression of Prlh in PrlhNTS neurons provided long-term protection from obesity in lean mice exposed to HFD following surgery (Fig. 4G, H). Here, PRLH is linked to obesity due to melanocortin 4 receptor deficiency.