Although psychostimulant addiction involves repeated use, a single dose of AMPH can induce enduring Str circuit changes, drug-dependent behavior and negative affective states, such as anhedonia, depression and anxiety (Vanderschuren et al., 1999; Koob and Le Moal, 2001; Xia et al., 2008; Kameda et al., 2011; Li et al., 2017; Jing et al., 2018; Rincón-Cortés et al., 2018; Jayanthi et al., 2020). The gene discussed is AMPH; the disease is major depressive disorder.