PAK1 and infection: When expressed in Hap1 cells Emerald-tagged PH-kinase, PH-CPP as well as CRIB-kinase were all successfully recruited by Salmonella after a 10-minute infection, confirming that an exogenous PH domain (and also the CRIB/AID of PAK1 itself) could successfully localise proteins to bacterial entry foci (S3D Fig).