It is not yet established if this PPI is necessary for alt-NHEJ function and therefore more investigation is needed.180SSA is another pathway of chemotheraputic interest,166,167,182 as Rad52 enhances the synthetic lethality of PARPi and BRCA deficiency.233,234 Thus, inhibitors of Rad52 were identified using fluorescence-quenching HTS assay for Rad52's ssDNA annealing activity, which selectively kill BRCA deficient cells.235. This evidence concerns the gene RAD52 and hyperinsulinemic hypoglycemia, familial, 4.