These results suggest that although acute Regorafenib treatment of hepatoblast-like cells initially creates a Wnt/β-catenin pathway-mediated increase in epithelial and stemness related markers, long-term regorafenib treatment of these cells leads to TGF-β pathway activation and the induction of mesenchymal cancer stem cell markers accompanied with an increased in vivo metastatic ability mediated by TGF-β pathway. Here, TGFB1 is linked to cancer.