Based on the public databases, 15 major CENPs, including CENPB, CENPC, CENPI, CENPJ, CENPL, CENPN, CENPO, CENPP, CENPQ, CENPR, CENPS, CENPT, CENPU, CENPV, and CENPX were analyzed by using bioinformatics tools and immunohistochemical staining to further investigate the gene expression patterns and prognostic value in HCC. This evidence concerns the gene CENPU and hepatocellular carcinoma.