In conclusion, rhein inhibited AlkB repair enzymes (AlkB, ALKBH2, and ALKBH3) in vitro and reduced cell resistance to MMS, and it was speculated that ALKBH2 and ALKBH3 enzymes may be effective pharmacological targets for overcoming tumor resistance to methylated anticancer drugs [86]. This evidence concerns the gene ALKBH3 and neoplasm.