Rare, predicted-pathogenic variants were found in 11 genes (ARID1B, CDON, CHD7, GLI1, GLI4, LHX3, LHX4, SIX1, SIX5, SIX6, SOX3) in 8 subjects with congenital hypopituitarism (62%) and in 3 genes (CHD7, LHX4, and WNT5A) in 4 subjects with NFSS (21%) (Table 3 and Supplementary Table 1). This evidence concerns the gene SIX5 and non-acquired combined pituitary hormone deficiency.