USP46 and major depressive disorder: They showed that Usp46 knockout mice exhibited shorter immobility times in the tail-suspension test, assessing depression-like behavior; reduced sucrose consumption in the sucrose preference test, assessing anhedonia-like symptoms; and lower locomotor activity levels in the open field test, assessing exploratory behavior and anxiety compared to wild type mice (33), which suggests the involvement of Usp46 in stress-related phenotypes.