In our study, SMG9 siRNA decreased the expression levels of Vimentin, N-cadherin, and β-catenin whereas increased the protein expression level of E-cadherin, indicating that SMG9 mediates HCC cell proliferation, apoptosis, migration and invasion via activating EMT and the Wnt/β-catenin signaling pathway. The gene discussed is SMG9; the disease is hepatocellular carcinoma.