Previous studies showed thatboth percentage and intensity of sperm DNA methylationby flow cytometry method were significantly lower ininfertile individuals with varicocele, compared to fertileindividuals, possibly due to hyperthermia (19, 20).Considering the fact that epigenetic status is influenced byenvironmental changes especially stressors (21), we aimedto access expression of DNMT1, DNMT3A and DNMT3Bat RNA and protein levels in sperm of individuals withvaricocele, compared to fertile individuals. Here, DNMT3A is linked to varicocele.