Theseobservations were contradictory to our expectation, sincewe previously reported lower degree of DNA methylationin spermatozoa of individuals with varicocele (20).Although DNMT1 is known as methyltransferasemaintenance, we were unable to observe any significantdifference in expression, percentage and intensity of thisenzymes between the two groups. Here, DNMT1 is linked to varicocele.