NOS1AP and Anxiety: However, in our study, anxiety-related behaviours in the open field (time in the centre: Fig. 3c, One-Way ANOVA, P=0·135), the light dark-box (light time: Fig. 3d, One-Way ANOVA, P=0·164; number of transitions: Fig. 3e, One-Way ANOVA, P=0·459), and the elevated zero maze (open arm time: Fig. 3f, One-Way ANOVA, P=0·858) were not affected by NOS1AP/NOS1AP396-503 overexpression.