PIK3CA and neoplasm: We also compared the PIK3CA assays results obtained on the cfDNA samples of a small subset of 4 patients to the the next-generation sequencing (NGS) results of the SAFIR02 study (NCT02299999) performed on tumor gDNA samples, and could confirm the presence of the same mutations for all of these patients, with the exception of a low frequency E545K mutation (MAF = 0.17%) found with the PIK3CA assays in plasma that could not be revealed by NGS in the tumor sample.