Our data demonstrating reductions in oxygen consumption at TN, but not at RT with CPF, are similar to observations in UCP1 null mice, which also only develop HFD-induced obesity when housed at TN; an effect attributed to impairments in BAT diet-induced thermogenesis7, We found that in HFD-fed mice housed at TN, CPF reduced Ucp1 mRNA and protein expression (Fig. 4a and b) and increased the size and number of lipid droplets (Fig. 4c) within the BAT. This evidence concerns the gene UCP1 and Obesity.