We found the conventional tumorigenesis-related WNT signaling pathway would have potential abilities of inducing CLM (Fig. 1B, Supplementary Fig. 1A), in CRC patient P5, P11, P15, APC mutation was only observed in the metastatic samples, and CCF analysis showed APC mutation was an early event in liver metastasis, indicating that APC would have a function of facilitating liver metastasis. The gene discussed is APC; the disease is colorectal carcinoma.