We explored the potential of two different combinations of TMs, and the results indicate that the combination of all 5 TMs, and the combination of CEA, CYFRA 21-1, and ProGRP, by virtue of having high values of sensitivity and specificity can work as viable supplements to the existing diagnostic modalities for LC diagnosis in primary and secondary health care centres in India. The gene discussed is GRP; the disease is laryngotracheoesophageal cleft.