EIF2S2 and infection: Next, 293 T cells were transfected with 50 nM eIF2S2 siRNA for 72 h or treated with 10 μM tomatidine for 2 h prior to transfection with 5 μg of TC-83 RNA containing the mutated stop codon or infection with the nano Luciferase expressing TC-83 virus (TaV-nLuc).