CASP3 and neoplasm: Figure 7 shows that the control and MBs + US groups did not show severe structural damage or apoptotic cells. Only minor structural damage and few apoptotic cells could be found in the TMZ and TMZ-lipo groups. In contrast, significant tumor necrosis and more apoptotic cells could be observed in the tumors of the group receiving TMZ-lipo combined with US-mediated BBB opening versus the control group (Figure 7A,B). Similarly, significantly more caspase-3-positive cells could be observed in the TMZ-lipo + US group compared with control, MBs + US, free TMZ, or TMZ-lipo groups (Figure 7A,C).