As an additional control, we performed experiments using 5 U/mL apyrase to deplete extracellular ATP of our cultures, finding that apyrase-treated AGS cells had no significant changes on VIM, CDH-1 nor ZO-1 mRNA levels compared to untreated AGS cells (Supplementary Figure S2B) Altogether, these results suggest a role for P2Y2R activation in promoting EMT on GC cell lines and stimulating epithelial phenotype on non-tumoral GES-1 cells. This evidence concerns the gene P2RY2 and gastric cancer.