To relate these EMT-induction effects to extracellular ATP levels on gastric cancer cell lines, we treated AGS cells with 5 U/mL apyrase for 48 h, and measured ZO-1, CDH-1, and VIM mRNA levels via qPCR, finding that apyrase-treated cells did not show any differences with untreated cells, suggesting that basal extracellular ATP plays a role on cell proliferation, but not on EMT in these particular experimental conditions. The gene discussed is VIM; the disease is gastric cancer.