To this end, we transduced Jurkat cells with SE and SE-IS2 (in-LTR) IDLVs at a multiplicity of infection (MOI = 4) in order to obtain homogenous eGFP positive cells prior to nucleofection using CRISPR/Cas9 to target the T cell receptor alpha chain (TRAC) coding region (See Figure S5). The gene discussed is TRAC; the disease is infection.