In contrast to depletion in TWIST or SNAIL, ZEB1 knockout has impair multiple stages of tumorigenesis including precursor lesion formation, tumor grading, invasion, and metastasis that clearly demonstrate a key role of ZEB1 in the in vivo tumor progression of pancreatic cancer from premalignant lesion towards metastasis [47]. Here, TWIST1 is linked to familial pancreatic carcinoma.