Western blot assays also indicated that expression of COX-2 protein increased upon MAI infection, as shown in Figure 2H. COX-2 expression level in MAI-infected human MDM also demonstrated a similar pattern, as shown in Figure 2I. Collectively, these data show that murine and human macrophages infected with NTM induce expression of pro-inflammatory mediators with increased expression of COX-2. The gene discussed is PTGS2; the disease is infection.