Finally, we co-transfected PUM1 sgRNAs and DDX5 overexpression vectors to SW480R and Caco-2R cells, the results of which exhibited that the overexpression of DDX5 could be relieved by PUM1 silencing (Figure 5), suggesting that DDX5 is critical in PUM1-mediated regulation in cetuximab-resistant colon cancer cells. Here, PUM1 is linked to malignant colon neoplasm.