Conversely, inhibition of KMT5B enzymatic activity with the selective drug A-196 (Bromberg et al., 2017) increased monomethylated and decreased dimethylated forms of H4K20 in stably-transfected clones, thus recapitulating the histone H4K20 methylation landscape of LN-229 GBM cell line (Figures 4C,D and Supplementary Figures 3C,D). Here, KMT5B is linked to glioblastoma.