Our results show that DT administration into NOD.Rag1–/– recipients of splenocytes from cohorts of adult NOD.DEREG females promotes overt diabetes in all recipients (Figure 3B), which correlated with an enrichment of effector/memory-type CD8+ T cells in the pLN (Figure 3C) but not scLN (data not shown). Here, RAG1 is linked to diabetes mellitus.