In particular, we observed: in active-TB a high proportion of monofunctional IFN-γ+/- IL-2- TNF-α+/- CD4+ T cells and triple functional IFN-γ+ IL-2+ TNF-α+ CD4+ T cells; in TBI, a great contribution of monofunctional IFN-γ+ IL-2- TNF-α- CD4+ T cells; in TBI-IMID, a high proportion of monofunctional IFN-γ+ IL-2- TNF-α- CD4+ T cells and triple functional IFN-γ+ IL-2+ TNF-α+ CD4+ T cells (Figure 6B). The gene discussed is IFNG; the disease is tuberculosis.