The concentration of CD40 in synaptosome fractions significantly increased in pooled cortical and hippocampal tissue in mice with tonic–clonic seizures (Racine’s score > 3) (Control: 68.81 ± 10.65 S.EM., n = 8 vs. Seizure: 165.60 ± 49.21 S.E.M., n = 6; p = 0.049) (Fig. 2D). Here, CD40 is linked to Seizure.