The purity of BMDCs was higher than 70% (range 65–80%) and their expression of CD11c, CD135, CD172a, CD370, CD205, MHC I, MHC II, CD86, CD80 and CD40 is shown in Fig. 2A. In a set of experiments, we analyzed whether 20 μg of pLL or 5 μg of HF were able to modulate the expression of costimulatory molecules (CD86, CD40 and CD80) and the major histocompatibility molecules (MHC I and MHC II). This evidence concerns the gene CD86 and hydrops fetalis.