While we detected elevated levels of tumor-infiltrating immune cells with tumor recognition potential such as CD8 T-cells, T follicular helper cells (Tfh), activated natural killer (NK) cells, and M1 macrophages in HS/CB patients when compared LS patients, the findings were not statistically significant (p > 0.10, two-sided Wilcoxon rank-sum test, Fig. 3E). This evidence concerns the gene CD8A and Leigh syndrome.