Confirming our results from the TCGA cohort, CTLA4 hypomethylation in primary ccRCC tissue at initial diagnosis was significantly associated with unfavorable EFS (HR 0.36 (95% CI 0.17 to 0.78), p<0.010) and OS (HR 0.35 (95% CI 0.16 to 0.75), p=0.007) in the UHB Non-ICB Cohort (figure 4B, C). The gene discussed is CTLA4; the disease is nonpapillary renal cell carcinoma.