Figure 2b shows the results of nuclear and cytoplasmic fractionation experiments. It is shown that TDP-43 accumulates in the cytosolic compartment of ALS lymphoblasts, while levels of nuclear TDP-43 decrease in comparison with control cells (Figure 2b). Tideglusib had no appreciable effects on control cells but was able to efficiently decrease the accumulation of cytosolic TDP-43 in ALS lymphoblasts, observing a trend to restore normal nuclear levels of this protein (Figure 2b). The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.