When Lrp1 was knocked out using the Nkx2-5-Cre [37] and Tie2-Cre [33] drivers simultaneously to investigate if Lrp1 in myocardial cells and in non-endocardium-derived cushion mesenchyme contributed synergistically to heart development, this resulted in 100% of the Tie2+/Cre/Nkx2-5+/Cre: Lrp1f/f mutant embryos exhibiting a CHD. Here, LRP1 is linked to coronary artery disorder.