Thus, for adoptive cell therapy with M2 microglia to treat a variety of neurodegenerative diseases that have chronic inflammatory backgrounds, it may be technically important to increase M2 microglial cell populations by in vitro astrocyte co-culture, GM-CSF priming, and the subsequent induction of M2 polarization with IL-4, IL-10, and SP. The gene discussed is IL4; the disease is neurodegenerative disease.