CASP3 and ischemia: Activation of caspase-3 and the fluorescence spectra of NucView-488 were recorded at 8 ± 4 min post-OGD-onset and we observed an increase in the fluorescence intensity of the probe that occurred within 40 min of ischemia, and a second peak in caspase-3 activation was seen 25 ± 10 min after reoxygenation (Figure 5B, OGD; Supplementary Figure S2, right recording—Control).