TLX3 and acute lymphoblastic leukemia: In T-ALL, T-cell leukemia homeobox protein 1-positive (TLX1+) patients express low levels of ASNS when compared with TLX3+ and TLX-negative patients, due to epigenetic silencing of ASNS by both DNA methylation and a decrease of active histone marks; in addition, promoter methylation of the ASNS gene correlated with ASNase sensitivity in both T-ALL cell lines and patient-derived xenografts [110].