Among the 54 molecular species (fatty acids with 14–30 carbons and 0–2 double bonds) for each GSL, 15 species of dihexosylceramide (Hex2Cer; LacCer), 19 species of GM3, 8 species of GM2, 4 species of GM1, 3 species of GD3, 18 species of Gb3, and 14 species of Gb4 were detected in fibroblasts from both X-ALD and control patients (Figure 2, Figure 3, Figure 4, Figure 5, Figure 6, Figure 7 and Figure 8). The gene discussed is CTSA; the disease is X-linked adrenoleukodystrophy.