To further understand the effects of sirolimus on the mTOR signaling pathway and p-cofilin, we isolated the LAM cells from LAM cell clusters (size between 100 μm to 70 μm) of LAM patients’ lung tissue (Figure 5a) and identified by positive HMB45 expression (Figure 5b). The gene discussed is TSC1; the disease is lymphangioleiomyomatosis.