In response to castration, Pb-Cre+/− Ptenfl/fl LSL-MYCN+/+ mice developed invasive tumours with metastatic features including epithelial-to-mesenchymal transition (EMT) (e.g., indicated by vimentin expression) and the expression of neural markers (e.g., NCAM1) [24]. Here, MYCN is linked to neoplasm.