REN and hyperlipidemia: To assess the contribution of elevated BP to plaque burden in the context of hyperlipidaemia, we stained aortas from ApoE−/− mice with Oil Red O. Lipid staining revealed lesions in the thoracic aortas, aortic arches, and secondary arterial branches of all HFD-fed AAV-Ren/Ang-transduced ApoE−/− mice and to a greater extent than in the vessels of similarly fed empty vector transduced ApoE−/− mice (Figure 2d,e).