In our analysis, atherosclerosis development in HFD-fed AAV-Ren/Ang-injected ApoE−/− mice on the C57BL/6J genetic background was accelerated compared to ApoE−/− mice injected with empty vector; however, AAV-Ren/Ang ApoE−/− and ApoE−/− mice on the atherogenic diet showed no differences in serum cholesterol and triglyceride or body weight, indicating that these parameters did not influence the observed difference in atherosclerosis development. The gene discussed is REN; the disease is atherosclerosis.