VPS35 and neurodegenerative disease: To investigate the Vps35 function in suppression of the development of neurodegenerative diseases, we generated LSL-Vps35-mCherry mice, which express VPS35-mCherry fusion protein in a Cre-dependent manner, since there is an insertion of a loxp-flanked “stop” sequence between the CAG promoter and Vps35-mCherry transgene (Figure S1A).