The specific antigen stimulus with HERV-K env 19–37 peptide produced significant differences in TNF-α expression between ALS patients and HCs (ALS vs. HCs, mean ± SD = 0.265 ± 0.158 vs. 0.127 ± 0.081; p = 0.002) (Figure 3c). This evidence concerns the gene TNF and amyotrophic lateral sclerosis.