Conversely, TNF-α was strongly expressed in HCs compared to ALS patients B cells, with a statistically significant difference for both peptides (ALS vs. HCs, mean ± SD = 0.0927 ± 0.038 vs. 0.386 ± 0.112; p = 0.0198) (ALS vs. HCs, mean ± SD = 0.206 ± 0.29 vs. 0.501 ± 0.06; p = 0.021), respectively (Figure 3a). The gene discussed is TNF; the disease is amyotrophic lateral sclerosis.