For the dataset of before and after anti-TNF treatment of RA patients, MAPK14 and MAPK1 activity levels were set to zero, and simulations turning the inputs sequentially active revealed that when setting either TNF, TGFB1, or IL6 on, all TFs are expressed (Figure 6a–c). The gene discussed is TGFB1; the disease is rheumatoid arthritis.