Literature analysis revealed that approximately 90% of CTNNB1 mutations in HCC occur in two hotspot regions, codons 32–37 and 41–45, accounting for 55% and 34% of all CTNNB1 mutations in HCC, respectively, as detailed in Supplemental Table S1 and summarized in Figure 1. This evidence concerns the gene CTNNB1 and hepatocellular carcinoma.