We conducted a retrospective analysis of liquid-based screening (LBS) tests results, including liquid-based cytology (LBC), 14 types of high-risk human papilloma virus (HRHPV14), and p16/Ki67 HSIL-risk biomarkers, along with histologic correlations in private-based opportunistic cervical cancer screening. The gene discussed is MKI67; the disease is cervical carcinoma.