The objective of this study was to develop a rapid and accurate diagnostic kit for CHF, which can be applied in clinical practice by selecting and recombining a new gene based on NT-proBNP and expressing it in Escherichiacoli. This process develops an antigen with high specificity that produces hybridomas and monoclonal antibodies while identifying optimal antigen-antibody reactivity via indirect enzyme-linked immunosorbent assay (ELISA). Here, NPPB is linked to congestive heart failure.